Detection of Laser-UV Microirradiation-induced DNA Photoiesions
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چکیده
A low-power laser-UV microbeam of wavelength 257 nm was used for microirradiation of a small part of the nucleus of Chinese hamster cells. Following fixation in interphflse or in the subsequent metaphase indirect immunofluorescent staining was performed with antiserum to photoproducts ofDNA treated with far UV ligh t. The results show that antibodies specific for UVirradiated DNA can be used for a direct detection of laser-UV microirradiation-induced DNA photoIesions. The potential usefulness of this method for investigation of the spatial arrangement of chromosomes in the interphase nucleus is discussed.
منابع مشابه
Laser-UV-microirradiation of Chinese hamster cells: the influence of the distribution of photolesions on unscheduled DNA synthesis.
Fibroblastoid Chinese hamster cells synchronized by mitotic selection were microirradiated in G1, using a low power laser-UV-microbeam (2 = 257 nm). The incident e?ergy was either concentrated on a small part of the nucleus (mode 1) or distributed over the whole nucleus (mode 11). Using the same incident UV energy, the local UV fluences were estimated to differ by two orders sf magnitude. Follo...
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تاریخ انتشار 2004